Difference between revisions of "Part:BBa K1321371"

 
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<partinfo>BBa_K1321371 short</partinfo>
 
<partinfo>BBa_K1321371 short</partinfo>
  
 
A LacI-promoter expression construct of super-folder GFP fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
 
A LacI-promoter expression construct of super-folder GFP fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
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At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
  
 
This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]
 
This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]

Revision as of 18:06, 17 October 2014

Linker-CBDcipA-Linker + sfGFP with LacI promoter

A LacI-promoter expression construct of super-folder GFP fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: IC14-sfGFP-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 383
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 383
    Illegal NgoMIV site found at 230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 962