Difference between revisions of "Part:BBa K1498001:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K1498001=== | ===Applications of BBa_K1498001=== | ||
| + | |||
| + | Lipase activity was observed following consultative expression in ''E. coli'' (DH5a) . This required co-expression of the ABC transporter BBa_K258008, to implement secretion of lipase into the extracellualr environment. ''E. coli'' harbouring BBa_K1498001 and BBa_K258008 were plated onto Rhodamine B (a fluorescent dye) plates supplemented with olive oil. Following overnight incubation a halo was seen around BBa_K1498001 and BBa_K258008 positive cells. Under UV light this effect was more apparent. In contrast this was not seen in Dh5a alone, which was used as a negative control. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:57, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1498001
Lipase activity was observed following consultative expression in E. coli (DH5a) . This required co-expression of the ABC transporter BBa_K258008, to implement secretion of lipase into the extracellualr environment. E. coli harbouring BBa_K1498001 and BBa_K258008 were plated onto Rhodamine B (a fluorescent dye) plates supplemented with olive oil. Following overnight incubation a halo was seen around BBa_K1498001 and BBa_K258008 positive cells. Under UV light this effect was more apparent. In contrast this was not seen in Dh5a alone, which was used as a negative control.
User Reviews
UNIQ7b0ff9f0d8ae3aa9-partinfo-00000000-QINU UNIQ7b0ff9f0d8ae3aa9-partinfo-00000001-QINU