Difference between revisions of "Part:BBa B0014"
Line 6: Line 6:
 
<partinfo>BBa_B0013 short</partinfo>
 
<partinfo>BBa_B0013 short</partinfo>
  
−
B0014  number 2 and by hand
+
B0014  number 2   third one by machine
  
 
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
 
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.

Revision as of 17:30, 26 April 2006

Designed by Reshma Shetty Entered: 2003-01-31

TE from coliphage T7 (+/-)

B0014 number 2 third one by machine

Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.

Usage and Biology

<img src="Include File TE.gif" width="413" height="246">

Above is a picture of the wildtype terminator. This terminator has been altered, see design notes. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]