Difference between revisions of "Part:BBa K1336003:Experience"
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===Applications of BBa_K1336003=== | ===Applications of BBa_K1336003=== | ||
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| + | <img src="https://static.igem.org/mediawiki/2014/7/7c/OResults_Bannero.jpg" width="100%" height="100%" alt="Results" /> | ||
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| + | <!-- Titles go in a <h1>TITLE GOES HERE</h1> and h1 is this biggest title and h6 is the smallest. all paragraphs go in <p>paragraph goes here</p> tags. Images go in as <img src="url of image here"> and to upload an image go to http://2014.igem.org/Special:Upload. Upload the image then click on the image which takes you to a page with only an image on it. The url of the image is the image you want to use. Use google and ask Lewis and Adam as much as you want--> | ||
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| + | <a href="http://2014.igem.org/Team:UCL/Science/Results/Deg"><span class="overlayx"><div class="resultsButton">Degradation</div></span></a> | ||
| + | <a href="http://2014.igem.org/Team:UCL/Science/Results/Sensor"><span class="overlayx"><div class="resultsButton">Azo-sensor</div></span></a> | ||
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| + | <h2>Degradation </h2> | ||
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| + | After having confirmed that Reactive Black 5 and Acid Orange 7 are not toxic and have no effect of ''E. coli'' DH5α in a wide range of concentrations, we set out to determine how the dye-decolorizing BioBrick BsDyP <a href="https://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> affected E. coli growth performance, both in standard LB medium and in media contaminated with RB5 and AO7 sulphonated azo-dyes. | ||
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| + | This was carried out by measuring bacterial OD at 680nm at regular intervals of 1 hour, in the different media. The choice of wavelength aims to reduce to a minimum the interference caused by the strong absorption of the dyes, while still measuring bacterial density. Although high-concentration RB5 still shows an absorption much higher than the other samples, the growth curve is not affected and so it allows to analyse how the presence of dyes might interfere with bacterial growth. The full protocol fot this assay can be found here (insert link). | ||
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| + | <img src="https://static.igem.org/mediawiki/2014/5/54/BsDyP_Figure_1.png "width="600" height="350"> | ||
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| + | <b>Figure 1 - <a href="https://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module preserves growth performance of E.coli DH5α in LB media. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module shows comparable growth the plasmid-free control in LB media. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:53, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1336003
Degradation
After having confirmed that Reactive Black 5 and Acid Orange 7 are not toxic and have no effect of ''E. coli'' DH5α in a wide range of concentrations, we set out to determine how the dye-decolorizing BioBrick BsDyP BBa_K1336003 affected E. coli growth performance, both in standard LB medium and in media contaminated with RB5 and AO7 sulphonated azo-dyes.This was carried out by measuring bacterial OD at 680nm at regular intervals of 1 hour, in the different media. The choice of wavelength aims to reduce to a minimum the interference caused by the strong absorption of the dyes, while still measuring bacterial density. Although high-concentration RB5 still shows an absorption much higher than the other samples, the growth curve is not affected and so it allows to analyse how the presence of dyes might interfere with bacterial growth. The full protocol fot this assay can be found here (insert link).
Figure 1 - BBa_K1336003 BsDyP Azo-degradation module preserves growth performance of E.coli DH5α in LB media. Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module shows comparable growth the plasmid-free control in LB media. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.
===User Reviews===