Difference between revisions of "Part:BBa J34804:Design"

 
 
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===Design Notes===
 
===Design Notes===
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===Source===
 
===Source===
  
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===References===
 
===References===
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Sreedevi Nallamsetty et al., Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro, ''Protein Expression and Purification'' 38 (2004) 108–115
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Rachel B. Kapust and David S. Waughn, Controlled Intracellular Processing of Fusion Proteins by TEV Protease, ''Protein Expression and Purification'' 19, 312–318 (2000)

Latest revision as of 08:58, 27 October 2006

TVMV protease


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 190
    Illegal XbaI site found at 573
    Illegal SpeI site found at 465
    Illegal PstI site found at 217
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 190
    Illegal SpeI site found at 465
    Illegal PstI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 190
    Illegal XhoI site found at 366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 190
    Illegal XbaI site found at 573
    Illegal SpeI site found at 465
    Illegal PstI site found at 217
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 190
    Illegal XbaI site found at 573
    Illegal SpeI site found at 465
    Illegal PstI site found at 217
    Illegal AgeI site found at 228
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

Sreedevi Nallamsetty et al., Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro, Protein Expression and Purification 38 (2004) 108–115 Rachel B. Kapust and David S. Waughn, Controlled Intracellular Processing of Fusion Proteins by TEV Protease, Protein Expression and Purification 19, 312–318 (2000)