Difference between revisions of "Part:BBa K1362054:Design"
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<partinfo>BBa_K1362054 short</partinfo> | <partinfo>BBa_K1362054 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | + | C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. | |
| − | + | The DNA sequence of the SsrA tag with overhangs was ordered as DNA oligos and cloned into (<partinfo>BBa_K1362052</partinfo>). | |
===References=== | ===References=== | ||
Latest revision as of 16:24, 17 October 2014
SsrA degradation tag as RFC[105] C-terminal insert
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 67
Design Notes
Source
C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. The DNA sequence of the SsrA tag with overhangs was ordered as DNA oligos and cloned into (BBa_K1362052).