Difference between revisions of "Part:BBa K1453000"

Revision as of 15:18, 17 October 2014

ssDsbA-mRFP-Lgt-TAL USB-His Tag

This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane.

Structure

The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000

In order to connect TAL protein designed by 2012 Freiburg iGEM team, the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.

When digested with BsmBI, this part can produce two sticky-ends that can bind TAL-Protein (Bba_K747000 to Bba_K747095)

After doing ligation with T4 Ligase, we experimentally validated this part`s function.

Sequencing result is followed: NNNCNNNGNGCATACCTCACTAAAGGGAACAAAAGCTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTT GATATCGAATTCCTGCAGCCCCCGCTCGAGTTAGTGGTGGTGGTGGTGGTGGGCCAGCGCGGGGTCCGGCCTCGAAAGCT GGGCCACGATTGACTCCAGTGCGGGTCTGCCCCCTCCGTTGGATGCAATAGCCACTACCTGCTCAGGCGTGAGCCCGTGG GCCTGACACAGCACCGGCAGCAGGCGCTGGACAGTCTCCAATGCCTGCTTACCGCCGCCGTTGCTCGCAATTGCAACCAC CTGTTCTGGGGTGAGACCATGTGCTTGACACAGCACCGGAAGCAAGCGCTGCACTGTCTCCAGCGCCTGTTTACCACCGC CATTGCTAGCGATGGCCACCACCTGTTCCGGGGTCAGGTTGAGGGGTGCTCCCGTGAGCGCATTGCGCCACGCGTGCACC GCCTCGACCGCCGTGACTCCTCCCCGCTTCGCGATCTTCAGCAACTGGCCCGTGTCCAGAGCTGCAGCACTACCACCTCC ACCACTTCCACCTCCACCACTACCTCCACCTCCTAAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCA TGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCGGGCACCGGTAAACTGC GCGTCGGGCTGGCGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCAT TGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCCAGCAGCAGCTCGTAAAGCTGTGATG GGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTA NGNNGCCAGGGAACAGCATGGNAAACGGGAAGTTCGGGTCAACGCGGCCCCCACAATTCACCGTTAATAAAGTTGCCCAG ACGCCCGGCACCAGACCAACGGGAATNNNGNGCGATAAAATCAGANACCTGNAGAGNANNTTTTANTACGGCGGGNNANA ATATTCATCANCNNCGAATACNNNNATTCAGGCNNNNNNNANNAANNNNCGNCCCNNTCCCA

Sequence and Features