Difference between revisions of "Part:BBa K1323022"

 
 
(4 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1323022 short</partinfo>
 
<partinfo>BBa_K1323022 short</partinfo>
  
−
TBA
+
sodA is a ribosome binding site that has been shown to be effective at generating fluorescent reporter signal in ''S.aureus/S.epidermidis'' hosts (Malone et al., 2007). sarA P1 [https://parts.igem.org/Part:BBa_K1323021 BBa_K1323021] promoter with sodA RBS generates higher level of protein expression than sarA P1 promoter with sarA RBS ribosome binding site (Malone et al., 2007). Sequence is taken from Malone et al., 2009 paper and synthesized by Biobasic.
 +
 
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
Line 12: Line 10:
 
<partinfo>BBa_K1323022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1323022 SequenceAndFeatures</partinfo>
  
 +
 +
 +
==References==
 +
 +
 +
Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent Reporters for Staphylococcus aureus. Journal of Microbiological Methods, 77(3): 251-260.
 +
 +
Franke, G. C., Dobinsky, S., Mack, D., Wang, C. J., Sobottka, I., Christner, M., Knobloch, J. K., Horstkotte, M. A., Aepfelbacher, M., & Rohde, H. (2007). Expression and functional characterization of gfpmut3.1 and its unstable variants in Staphylococcus epidermidis. Journal of Microbiological methods 71, 123-132.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:02, 17 October 2014

Ribosome binding site from the sodA Staphylococcus aureus gene

sodA is a ribosome binding site that has been shown to be effective at generating fluorescent reporter signal in S.aureus/S.epidermidis hosts (Malone et al., 2007). sarA P1 BBa_K1323021 promoter with sodA RBS generates higher level of protein expression than sarA P1 promoter with sarA RBS ribosome binding site (Malone et al., 2007). Sequence is taken from Malone et al., 2009 paper and synthesized by Biobasic.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent Reporters for Staphylococcus aureus. Journal of Microbiological Methods, 77(3): 251-260.

Franke, G. C., Dobinsky, S., Mack, D., Wang, C. J., Sobottka, I., Christner, M., Knobloch, J. K., Horstkotte, M. A., Aepfelbacher, M., & Rohde, H. (2007). Expression and functional characterization of gfpmut3.1 and its unstable variants in Staphylococcus epidermidis. Journal of Microbiological methods 71, 123-132.