Difference between revisions of "Part:BBa K1524100"
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All you have to do is to insert your target gene sequence upside down using NcoI and XhoI restriction enzymes.
 
All you have to do is to insert your target gene sequence upside down using NcoI and XhoI restriction enzymes.
 
Add IPTG, and your target will be repressed!
 
Add IPTG, and your target will be repressed!
−
Notice that this stem sequence has a resistance to PCR, especially sequencing PCR.
+
Notice that this stem sequence has a resistance to PCR, especially sequencing PCR. And notice that its vector is pSB1A3 becaouse 1C3 vector has both NcoI site and XhoI site.
  
 
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<!-- Add more about the biology of this part here

Revision as of 14:53, 17 October 2014

H-stem expression vector

This device provides you an easy and strong method to silence mRNA using anti-sense RNA. All you have to do is to insert your target gene sequence upside down using NcoI and XhoI restriction enzymes. Add IPTG, and your target will be repressed! Notice that this stem sequence has a resistance to PCR, especially sequencing PCR. And notice that its vector is pSB1A3 becaouse 1C3 vector has both NcoI site and XhoI site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 477
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 342
    Illegal SpeI site found at 478
    Illegal PstI site found at 492
    Illegal NotI site found at 485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 300
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 478
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 478
    Illegal PstI site found at 492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 239