Difference between revisions of "Part:BBa K1398003"

Revision as of 14:44, 17 October 2014

NemA (Inducible Construct)

A construct created by the 2014 Exeter iGEM team to degrade TNT and nitroglycerin. The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. The protein has been codon-optimised for expression in E. coli.

The Exeter 2014 team used Raman spectroscopy to show the breakdown of Nitroglycerin by NemA:

The Exeter 2014 team also showed that NemA cannot perform this catalytic function without cofactors: FMN, NADH and NADPH.

Analysis of these data allowed the kinetic parameters of Km and Vmax of NemA for nitroglycerin to be calculated as 6 M and 21 umol per mg per minute, respectively.

Full article at: http://2014.igem.org/Team:Exeter/EnzymeValidation

Sequence and Features