Difference between revisions of "Part:BBa K1398001"
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The construct contains the coding sequence for XenB (BBa_K1398000), an enzyme with the capability to degrade nitro- groups in chemicals. | The construct contains the coding sequence for XenB (BBa_K1398000), an enzyme with the capability to degrade nitro- groups in chemicals. | ||
The construct also contains a Lactose inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator of BBa_B0010 and BBa_B0012. | The construct also contains a Lactose inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator of BBa_B0010 and BBa_B0012. | ||
| − | The protein has been codon-optimised for expression in E. coli by DNA2.0 More information can be found here: https://www.dna20.com/services/genegps. | + | The protein has been codon-optimised for expression in E. coli by DNA2.0. More information can be found here: https://www.dna20.com/services/genegps. |
Latest revision as of 14:41, 17 October 2014
XenB (Inducible Construct)
A construct created to degrade TNT and nitroglycerin. The construct contains the coding sequence for XenB (BBa_K1398000), an enzyme with the capability to degrade nitro- groups in chemicals. The construct also contains a Lactose inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator of BBa_B0010 and BBa_B0012. The protein has been codon-optimised for expression in E. coli by DNA2.0. More information can be found here: https://www.dna20.com/services/genegps.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]