Difference between revisions of "Part:BBa B0013"

 
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<SPAN style='position:absolute;font-size:90%;position:relative;top:-15px'>Designed by <partinfo>B0011 designer</partinfo></SPAN>
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<SPAN style='position:absolute;font-size:90%;position:relative;top:-15px'>
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<SPAN style='position:absolute;float:right;font-size:90%;position:relative;top:-15px'>Entered: <partinfo>B0011 entrydate</partinfo></SPAN>
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Designed by <partinfo>BBa_B0013 designer</partinfo></SPAN>
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<SPAN style='position:absolute;float:right;font-size:90%;position:relative;top:-15px'>
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Entered: <partinfo>BBa_B0013 entrydate</partinfo></SPAN>
 
__NOTOC__
 
__NOTOC__
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<partinfo>B0012 short</partinfo>
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<partinfo>BBa_B0013 short</partinfo>
  
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Bidirectional transcriptional terminator consisting of a 22bp stem-loop
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Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
  
 
===Usage and Biology===
 
===Usage and Biology===
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Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the
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<img src="'''Include File TE.gif'''" width="413" height="246">    <p>Above is a picture of the wildtype terminator. This terminator has been altered,      see design notes.
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stem loop. It is also found between two coding regions that point towards each other.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>B0012 SequenceAndFeatures</partinfo>
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<partinfo>BBa_B0013 SequenceAndFeatures</partinfo>
  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>B0012 parameters</partinfo>
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<partinfo>BBa_B0013 parameters</partinfo>

Revision as of 12:34, 25 April 2006

Designed by Reshma Shetty Entered: 2003-01-31

TE from coliphage T7 (+/-)

Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.

Usage and Biology

<img src="Include File TE.gif" width="413" height="246">

Above is a picture of the wildtype terminator. This terminator has been altered, see design notes. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]