Difference between revisions of "Part:BBa K1509002:Design"
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P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ | P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ | ||
And the PCR runs under the settings as follows: | And the PCR runs under the settings as follows: | ||
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| + | Temperature： 96℃ 94℃ 50℃ 72℃ 72℃ | ||
| + | Time： 15min 40sec 60sec 4min 7min | ||
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| + | Number of cycle： 1 30 1 | ||
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| + | Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25. | ||
==='''Sourse'''=== | ==='''Sourse'''=== | ||
It’s origin is one strain named as ''Bacillus sp''. F2, whose flocculent ability can reach 84%. | It’s origin is one strain named as ''Bacillus sp''. F2, whose flocculent ability can reach 84%. | ||
Revision as of 13:55, 17 October 2014
Design Notes
It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below: P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’ P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ And the PCR runs under the settings as follows:
Temperature： 96℃ 94℃ 50℃ 72℃ 72℃
Time： 15min 40sec 60sec 4min 7min
Number of cycle： 1 30 1
Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
Sourse
It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.