Difference between revisions of "Part:BBa K1462090"

Latest revision as of 13:27, 17 October 2014

Gal1+cox6+GFP+ADH1

To verify the localization function of CoxVI, the C-terminus of CoxVI was fused with GFP. We adopted fluorescence test to have a visible look at where the GFP is located. The following microscope images were captured(Figure 1). We can observe that the green fluorescence concentrates in a specific area , instead of diffusing homogeneously in the cytosol.The reult proves that CoxVI can perform its function properly

Figure 1.COXVI function verification GFP fused with COXVI is concentrated in a specific area of the cytoplasm,while GFP without COXVI diffuses in the cytoplasm of the cell.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1325

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 <partinfo>BBa_K1462090 short</partinfo>
-To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.We adopted fluorescence test to have a visible look at where the fusion protein is located. The following microscope images were obtained. It was observed that green fluorescence concentrated in mitochondria stained by a specific stain, instead of diffusing in the cytosol.The reult  proves that CoxVI can locate GFP into the mitochondrial matrix.
+To verify the localization function of CoxVI, the C-terminus of CoxVI was fused with GFP. We adopted fluorescence test to have a visible look at where the GFP is located. The following microscope images were captured(Figure 1). We can observe that the green fluorescence concentrates in a specific area , instead of diffusing homogeneously in the cytosol.The reult proves that CoxVI can perform its function properly
-[[File:G6GT2.jpg|100px|left|frame|Figue1.GFP is targed to the mitochondria]]
+[[File:G6GT.jpg|200px|left|frame|<b>Figure 1.COXVI function verification</b> GFP fused with COXVI is concentrated in a specific area of the cytoplasm,while GFP without COXVI diffuses in the cytoplasm of the cell. ]]