Difference between revisions of "Part:BBa K1462090:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | + | To verify the localization function of CoxVI, the C-terminus of CoxVI was fused with GFP. We adopted fluorescence test to have a visible look at where the GFP is located. The following microscope images were captured(Figure 1). We can observe that the green fluorescence concentrates in a specific area , instead of diffusing homogeneously in the cytosol.The reult proves that CoxVI can perform its function properly | |
| + | [[File:G6GT.jpg|200px|left|frame|<b>Figure 1.COXVI function verification</b> GFP fused with COXVI is concentrated in a specific area of the cytoplasm,while GFP without COXVI diffuses in the cytoplasm of the cell. ]] | ||
Latest revision as of 13:27, 17 October 2014
Gal1+cox6+GFP+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1325
Design Notes
To verify the localization function of CoxVI, the C-terminus of CoxVI was fused with GFP. We adopted fluorescence test to have a visible look at where the GFP is located. The following microscope images were captured(Figure 1). We can observe that the green fluorescence concentrates in a specific area , instead of diffusing homogeneously in the cytosol.The reult proves that CoxVI can perform its function properly
Figure 1.COXVI function verification GFP fused with COXVI is concentrated in a specific area of the cytoplasm,while GFP without COXVI diffuses in the cytoplasm of the cell.