Difference between revisions of "Part:BBa K1404006:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
<html>
 
<html>
−
If you intend to use this part, be aware that CsgA might be toxic to <i>E. coli</i> cells in large quantities. We were unable to clone <i>csgA</i> in front of the strong promoter <a href="https://parts.igem.org/Part:BBa_J23119">J23119</a>J23119 or the weaker promoter <a href="https://parts.igem.org/Part:BBa_J23114">J23114</a>
+
If you intend to use this part, be aware that CsgA might be toxic to <i>E. coli</i> cells in large quantities. We were unable to clone <i>csgA</i> in front of the strong promoter <a href="https://parts.igem.org/Part:BBa_J23119">J23119</a> or the weaker promoter <a href="https://parts.igem.org/Part:BBa_J23114">J23114</a>
 
</html>
 
</html>
  

Revision as of 13:07, 17 October 2014


p70-CsgA, curli generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you intend to use this part, be aware that CsgA might be toxic to E. coli cells in large quantities. We were unable to clone csgA in front of the strong promoter J23119 or the weaker promoter J23114

Source

temp

References