Difference between revisions of "Part:BBa K1362054:Design"
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===Source=== | ===Source=== | ||
| − | + | C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. | |
| − | + | The DNA sequence of the SsrA tag with overhangs was ordered as a DNA oligo and cloned into (<partinfo>BBa_K1362052</partinfo>). | |
===References=== | ===References=== | ||
Revision as of 13:03, 17 October 2014
SsrA degradation tag as RFC[105] C-terminal insert
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 67
Design Notes
Source
C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. The DNA sequence of the SsrA tag with overhangs was ordered as a DNA oligo and cloned into (BBa_K1362052).