Difference between revisions of "Part:BBa K1509000"

Revision as of 13:00, 17 October 2014

Coding for the trans-acting regulator SmtB

In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus elongates PCC7942. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn²⁺ and Cd²⁺. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn²⁺ there are four potential metal binding sites on protein SmtB as known as α3, α3N, α5 and α5C ^[9], different from Zn²⁺ resistance mechanisms, Cd²⁺ binds SmtB at the site α3N ^[10]. These two mechanisms both cause the elevated expression of smtA.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 349
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 260
Illegal PstI site found at 349
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 349
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 349
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1509000 short</partinfo>
-In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented  octanucleotides(5&#8242;-GCGATCGC-3&#8242;) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.
+In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from ''Synechococcus elongates'' PCC7942. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn<sup>2+</sup> and Cd<sup>2+</sup>. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn<sup>2+</sup> there are four potential metal binding sites on protein SmtB as known as α3, α3N, α5 and α5C <sup>[9]</sup>, different from Zn<sup>2+</sup> resistance mechanisms, Cd<sup>2+</sup> binds SmtB at the site α3N <sup>[10]</sup>. These two mechanisms both cause the elevated expression of smtA.
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