Difference between revisions of "Part:BBa K1415207"
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<partinfo>BBa_K1415207 short</partinfo> | <partinfo>BBa_K1415207 short</partinfo> | ||
− | We | + | To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli. |
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[[File:ALLAS.png|thumb|left|900px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(AS) + BFP + Term.]] | [[File:ALLAS.png|thumb|left|900px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(AS) + BFP + Term.]] | ||
[[File:BLAS.png|thumb|left|1200px|'''Fig.1-1''' Fig.1-2 The bluelight test of our E.coli]] | [[File:BLAS.png|thumb|left|1200px|'''Fig.1-1''' Fig.1-2 The bluelight test of our E.coli]] |
Revision as of 12:51, 17 October 2014
Pcons+B0034+PBAN(Adoxophyes sp.)+B0034+BFP+J61048
To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 903
- 1000COMPATIBLE WITH RFC[1000]