Difference between revisions of "Part:BBa K1415205"
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<partinfo>BBa_K1415205 parameters</partinfo>
 
<partinfo>BBa_K1415205 parameters</partinfo>
 
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[[File:ALLSL.png|1200px|link=|frameless|left]]
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[[File:ALLSL.png|thumb|right|1200px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(SL) + BFP + Term. ]]
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[[File:BLSL.png|thumb|right|1200px|'''Fig.1-2''' The growth curve of E.coli containing Pcons + RBS +  PBAN(SL) + RBS + BFP + Ter plasmid (control is the competent cells which can not emit blue light). ]]
  
 
[[File:MDSL.png|thumb|left|1200px|'''Fig.1-3'''Modeling result of Pcons + RBS + PBAN(SL) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(SL) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias. ]]
 
[[File:MDSL.png|thumb|left|1200px|'''Fig.1-3'''Modeling result of Pcons + RBS + PBAN(SL) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(SL) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias. ]]

Revision as of 12:41, 17 October 2014

Pcons+B0034+PBAN(Spodoptera litura)+B0034+BFP+J61048

To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 909
  • 1000
    COMPATIBLE WITH RFC[1000]



Fig.1-1 Biobrick of Pcons + RBS + PBAN(SL) + BFP + Term.
Fig.1-2 The growth curve of E.coli containing Pcons + RBS + PBAN(SL) + RBS + BFP + Ter plasmid (control is the competent cells which can not emit blue light).
Fig.1-3Modeling result of Pcons + RBS + PBAN(SL) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(SL) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.