Difference between revisions of "Part:BBa C0083:Experience"

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We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562017</partinfo>). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in <i>E. coli</i> using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.
 
We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562017</partinfo>). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in <i>E. coli</i> using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.
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<partinfo>BBa_K190019 AddReview </partinfo>
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<I>iGEM Nagahama_2014</I>
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BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removes LVA tag in BBa_C0083 resulting to new parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342002 BBa_K1342002]
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And we detected and quantified of aspartic acid synthesized by  <i>
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E. coli</i>JM109/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342001 BBa_K1342001] by TLC.
 
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Latest revision as of 12:38, 17 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_C0083

User Reviews

UNIQ81069e99f6fab59e-partinfo-00000000-QINU


•••

iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562017). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.

UNIQ81069e99f6fab59e-partinfo-00000003-QINU


Review number not understood iGEM Nagahama_2014

BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removes LVA tag in BBa_C0083 resulting to new parts BBa_K1342002 And we detected and quantified of aspartic acid synthesized by E. coliJM109/BBa_K1342001 by TLC.

UNIQ81069e99f6fab59e-partinfo-00000005-QINU