Difference between revisions of "Part:BBa K1331004"
Line 6: Line 6:
 
It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.  
 
It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.  
  
−
The coding sequence of our part is similar with [[Part:BBa_K317999]][https://parts.igem.org/Part:BBa_K317999] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
+
The coding sequence of our part is similar with [https://parts.igem.org/Part:BBa_K317999 Part:BBa_K317999] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
−
RhlB together with [[rhlA]][https://parts.igem.org/Part:BBa_K1331001] encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.
+
RhlB together with [https://parts.igem.org/Part:BBa_K1331001 rhlA] encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:29, 17 October 2014

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit B.

It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.

The coding sequence of our part is similar with Part:BBa_K317999 designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.


Usage and Biology

RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387