Difference between revisions of "Part:BBa K1453000"
(→‎Structure)
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The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000  
 
The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000  
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In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
+
In order to be used cooperatively with TAL-Protein DiRepeats (BBa_K747000 to BBa_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
  
  

Revision as of 12:19, 17 October 2014

ssDsbA-mRFP-Lgt-TAL USB-His Tag

This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane.


Structure

Part%EF%BC%9ABBa_K1453000.png

The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 In order to be used cooperatively with TAL-Protein DiRepeats (BBa_K747000 to BBa_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 612
    Illegal AgeI site found at 724
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1158