Difference between revisions of "Part:BBa K1326003"
 
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Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule.
 
Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule.
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Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide.  
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Light-dependent protochlorophyllidereductase ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080007 POR]), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide.  
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DVR1 is a chloroplast transit peptide.
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080012 DVR1] is a chloroplast transit peptide.
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Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain
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Plant geranylgeranylhydrogenase ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080008 ChlP]) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain
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Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.
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Chlorophyllsynthetase ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080009 ChlG]) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.
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<br><br>
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[[File:pSB1C3_Operon 3.png]]
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<br>'''''Figure 1:''''' Operon 3 in the chlorophyll biosynthesis pathway.
 
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<!-- Add more about the biology of this part here
 
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===Functional Parameters===
 
===Functional Parameters===
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[[File:GEL_Operon 3 Chlorophyll a]]
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[[File:GEL_Operon 3 Chlorophyll a.png]]
 
<br>'''''Figure 1:''''' Single and double restriction digests of Operon 3. Total plasmid length is 7.10 kB, as can be seen by the dark band in the single digest lane. Double digest lane shows the operon fragment at 4.90 kB, and the BioBrick plasmid backbone at 2.20.
 
<br>'''''Figure 1:''''' Single and double restriction digests of Operon 3. Total plasmid length is 7.10 kB, as can be seen by the dark band in the single digest lane. Double digest lane shows the operon fragment at 4.90 kB, and the BioBrick plasmid backbone at 2.20.

Latest revision as of 12:18, 17 October 2014

Plac + POR + DVR1 + ChlP + ChlG

Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule. Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide. DVR1 is a chloroplast transit peptide. Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.

PSB1C3 Operon 3.png
Figure 1: Operon 3 in the chlorophyll biosynthesis pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 808
    Illegal BglII site found at 4090
    Illegal BglII site found at 4579
    Illegal BamHI site found at 1571
    Illegal BamHI site found at 3859
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 347
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2461


Functional Parameters

GEL Operon 3 Chlorophyll a.png
Figure 1: Single and double restriction digests of Operon 3. Total plasmid length is 7.10 kB, as can be seen by the dark band in the single digest lane. Double digest lane shows the operon fragment at 4.90 kB, and the BioBrick plasmid backbone at 2.20.