Difference between revisions of "Part:BBa K1462110:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Erg10 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins. | This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Erg10 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins. | ||
| − | [[File: | + | [[File:GEG2.jpg|200px|left|frame|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that Erg10 is expressed in the cytoplasm]] |
Latest revision as of 12:09, 17 October 2014
Gal1+Erg10+GFP+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1252
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2402
Design Notes
This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Erg10 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Figure 1. Enzymes located in cytoplasm The fluorescence indicates that Erg10 is expressed in the cytoplasm