Difference between revisions of "Part:BBa K1321116"

 
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<partinfo>BBa_K1321116 short</partinfo>
 
<partinfo>BBa_K1321116 short</partinfo>
  
CBD10+PhytoC  with LacI
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A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
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At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
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This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
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Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
  
 
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Revision as of 12:05, 17 October 2014

Linker-cipA-linker + phytochelatin (PC) EC20 with LacI promoter

A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 383
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 383
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]