Difference between revisions of "Part:BBa K1331001"
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<partinfo>BBa_K1331001 short</partinfo> | <partinfo>BBa_K1331001 short</partinfo> | ||
| − | This part encodes rhamnosyltransferase I subunit A. Another subunit is [[rhlB]][https://parts.igem.org/Part:BBa_K1331004 | + | This part encodes rhamnosyltransferase I subunit A. Another subunit is [[rhlB]][https://parts.igem.org/Part:BBa_K1331004]. |
It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA. | It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA. | ||
Revision as of 11:54, 17 October 2014
Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6
This part encodes rhamnosyltransferase I subunit A. Another subunit is rhlB[1].
It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.
The coding sequence of our part is similar with Part:BBa_K317998[2] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
Usage and Biology
RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478