Difference between revisions of "Part:BBa K824008:Experience"
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Oct. 2014: Part K1320000 contains the same cadmium responsive promoter and GFP but includes sensitivity tuners. It is far more sensitive than this part (2.5mM Cd). | Oct. 2014: Part K1320000 contains the same cadmium responsive promoter and GFP but includes sensitivity tuners. It is far more sensitive than this part (2.5mM Cd). | ||
| − | We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. The graph below shows our results. | + | |
| + | Oct. 2012: We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. The graph below shows our results. | ||
<center>https://static.igem.org/mediawiki/2012/8/8c/Cadgraphuse.jpg</center> | <center>https://static.igem.org/mediawiki/2012/8/8c/Cadgraphuse.jpg</center> | ||
Latest revision as of 11:48, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K824008
User Reviews
UNIQfe6ecec05a1d3a72-partinfo-00000000-QINU UNIQfe6ecec05a1d3a72-partinfo-00000001-QINU Oct. 2014: Part K1320000 contains the same cadmium responsive promoter and GFP but includes sensitivity tuners. It is far more sensitive than this part (2.5mM Cd).
Oct. 2012: We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. The graph below shows our results.
