Difference between revisions of "Part:BBa K1351031"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1351031 short</partinfo> | <partinfo>BBa_K1351031 short</partinfo> | ||
| − | "This part was generated in a modified version of | + | "This part was generated in a modified version of RFC10, and has the following prefix and suffix: |
{| | {| | ||
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV: | |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: | ||
| − | |<span style= | + | |<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span> |
|- | |- | ||
|suffix with AgeI, SpeI, NotI and PstI: | |suffix with AgeI, SpeI, NotI and PstI: | ||
| − | |<span style= | + | |<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span> |
|} | |} | ||
Sites of restriction enzymes generating compatible overhangs have the same color: | Sites of restriction enzymes generating compatible overhangs have the same color: | ||
| − | <span style= | + | <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 11:47, 17 October 2014
LMU Bacillus RBS collection 4
"This part was generated in a modified version of RFC10, and has the following prefix and suffix:
| prefix with EcoRI, NotI, XbaI, SD and NgoMIV: | GAATTCGCGGCCGCTTCTAGATGGCCGGC |
| suffix with AgeI, SpeI, NotI and PstI: | ACCGGTTAATACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]