Difference between revisions of "Part:BBa K1331004"
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This part encodes rhamnosyltransferase I subunit B. | This part encodes rhamnosyltransferase I subunit B. | ||
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It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB. | It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB. | ||
The coding sequence of our part is similar with [[Part:BBa_K317999]][https://parts.igem.org/Part:BBa_K317999] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. | The coding sequence of our part is similar with [[Part:BBa_K317999]][https://parts.igem.org/Part:BBa_K317999] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | RhlB together with [[rhlA]][https://parts.igem.org/Part:BBa_K1331001] encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1331004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1331004 SequenceAndFeatures</partinfo> | ||
Revision as of 11:45, 17 October 2014
Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
This part encodes rhamnosyltransferase I subunit B. It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB. The coding sequence of our part is similar with Part:BBa_K317999[1] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
Usage and Biology
RhlB together with rhlA[2] encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 871 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 387