Difference between revisions of "Part:BBa K1331001"

Revision as of 11:42, 17 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit A. It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA. The coding sequence of our part is similar with Part:BBa_K317998 [1] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478

@@ Line 5: / Line 5: @@
 It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.
 The coding sequence of our part is similar with [[Part:BBa_K317998]][https://parts.igem.org/Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
 ===Usage and Biology===
 RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.
 <span class='h3bb'>Sequence and Features</span>