Difference between revisions of "Part:BBa K1331001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | A mutation has been done to remove the PstI restriction site contained in the coding sequence. | + | A mutation has been done to remove the PstI restriction site contained in the coding sequence without changing the amino acid sequence. |
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===Source=== | ===Source=== | ||
| − | ''Pseudomonas aeruginosa'' | + | ''Pseudomonas aeruginosa'' SQ6 |
===References=== | ===References=== | ||
Latest revision as of 11:26, 17 October 2014
Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478
Design Notes
A mutation has been done to remove the PstI restriction site contained in the coding sequence without changing the amino acid sequence.
Source
Pseudomonas aeruginosa SQ6