Difference between revisions of "Part:BBa K1331004"

Revision as of 11:21, 17 October 2014

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit B. RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB. The coding sequence of our part is similar with Part:BBa_K317999 [1] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 871
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 387

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1331004 short</partinfo>
-RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part includes a part of upstream noncoding gene and a part of downstreanm noncoding gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
+This part encodes rhamnosyltransferase I subunit B.
+RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.
+It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.
+The coding sequence of our part is similar with [[Part:BBa_K317999]][https://parts.igem.org/Part:BBa_K317999] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
 <!-- Add more about the biology of this part here