Difference between revisions of "Part:BBa J01008:Experience"
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<I>HZAU-China 2014</I> | <I>HZAU-China 2014</I> | ||
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| − | We want to know under the participation of taRNA and crRNA, whether can reduce leakage of lac promoter’s expression. So we did some experiment to text. First, built the tow devices and from top to bottom Numbers for 1 2 , and then, respectively transform the | + | We want to know under the participation of taRNA and crRNA, whether can reduce leakage of lac promoter’s expression. So we did some experiment to text. First, built the tow devices and from top to bottom Numbers for 1 2 , and then, respectively transform the two plasmid into BL21 competent cell. Pick single colonies. Add 5ml LB liquid and incubate at 37 Celsius degree for 8 hours. Secondly, add 5ul of E.coli liquid cultural medium from each tube to two new tubes named A, B respectively with 5ml M9 broth in it. Add 0.1ul IPTG to all A tubes (1A, 2A, ) while add nothing to all B tubes (1B, 2B, ). Incubating at 37 Celsius degree for 4 hours. Lastly, easuring the fluorescent degree by utilizing the enzyme-labeled instrument. |
Conclusion : riboregulators ensure much lower leakage of the promoters. | Conclusion : riboregulators ensure much lower leakage of the promoters. | ||
| − | [[File:HZAU2014-图片1.jpg| | + | [[File:HZAU2014-图片1.jpg|380px|left]] |
| − | [[File:HZAU2014-图片2.jpg| | + | [[File:HZAU2014-图片2.jpg|400px|right]] |
| − | [[File:HZAU2014-ribo.jpg| | + | [[File:HZAU2014-ribo.jpg|800px]] |
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Latest revision as of 11:18, 17 October 2014
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HZAU-China 2014 |
We want to know under the participation of taRNA and crRNA, whether can reduce leakage of lac promoter’s expression. So we did some experiment to text. First, built the tow devices and from top to bottom Numbers for 1 2 , and then, respectively transform the two plasmid into BL21 competent cell. Pick single colonies. Add 5ml LB liquid and incubate at 37 Celsius degree for 8 hours. Secondly, add 5ul of E.coli liquid cultural medium from each tube to two new tubes named A, B respectively with 5ml M9 broth in it. Add 0.1ul IPTG to all A tubes (1A, 2A, ) while add nothing to all B tubes (1B, 2B, ). Incubating at 37 Celsius degree for 4 hours. Lastly, easuring the fluorescent degree by utilizing the enzyme-labeled instrument. Conclusion : riboregulators ensure much lower leakage of the promoters.  
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