Difference between revisions of "Part:BBa K1331001"

Revision as of 11:09, 17 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit A.RhlA together with rhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. It includes the whole coding sequence and a part of downstream noncoding gene of RhlA. The coding sequence is similar with Part:BBa_K317998 [1] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478

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 This part encodes rhamnosyltransferase I subunit A.RhlA together with rhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''.
 It includes the whole coding sequence and a part of downstream noncoding gene of RhlA.
-The coding sequence is similar with[[Part:BBa_K317998]][https://parts.igem.org/Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
+The coding sequence is similar with [[Part:BBa_K317998]][https://parts.igem.org/Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
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