Difference between revisions of "Part:BBa K1462090"
Line 5: Line 5:
 
[[File:G6GT2.jpg|100px|left|frame|Figue1.GFP is targed to the mitochondria]]
 
[[File:G6GT2.jpg|100px|left|frame|Figue1.GFP is targed to the mitochondria]]
  
−
===Source===
 
−
 
−
COXVI is obtained from genome of saccharomyces cerevisiae, the other parts is from the kit provided by official
 
−
 
−
===References===
 
−
Ammy C. Maarse1 et al, Subunit IV of yeast cytochrome c oxidase: cloning and nuicleotide sequencing of the gene and partial amino acid sequencing of the mature protein,The EMBO Journal vol.3 no.12 pp.2831 -2837, 1984
 
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->

Revision as of 09:48, 17 October 2014

Gal1+cox6+GFP+ADH1

To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.We adopted fluorescence test to have a visible look at where the fusion protein is located. The following microscope images were obtained. It was observed that green fluorescence concentrated in mitochondria stained by a specific stain, instead of diffusing in the cytosol.The reult proves that CoxVI can locate GFP into the mitochondrial matrix.

Figue1.GFP is targed to the mitochondria



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1325