Difference between revisions of "Part:BBa K1462240:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Ccr,originated from Streptomyces collinus, expresses butyryl-CoA dehydrogenase, which converts CrCoA to BtCoA, the fourth step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with AdhE2(BBa_K1462250) in plasmid 352 ,which is uracil auxotroph. | |
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− | + | [[File:pathway.png|100px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]] |
Latest revision as of 09:30, 17 October 2014
Gal1+Ccr+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1203
Design Notes
The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Ccr,originated from Streptomyces collinus, expresses butyryl-CoA dehydrogenase, which converts CrCoA to BtCoA, the fourth step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with AdhE2(BBa_K1462250) in plasmid 352 ,which is uracil auxotroph.