Difference between revisions of "Part:BBa K1415207"
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We use this part as reporter gene, it can check whether our PBAN works and quantify its expression. | We use this part as reporter gene, it can check whether our PBAN works and quantify its expression. | ||
| + | [[File:ALLAS.png|thumb|left|900px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(AS) + BFP + Term.]] | ||
| + | [[File:BLAS.png|thumb|left|1200px|'''Fig.1-1''' Fig.1-2 The bluelight test of our E.coli]] | ||
| + | [[File:MDAS.png|thumb|left|1200px|'''Fig.1-1''' Modeling result of Pcons + RBS + PBAN(AS) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AS) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.]] | ||
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<partinfo>BBa_K1415207 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1415207 SequenceAndFeatures</partinfo> | ||
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Revision as of 08:52, 17 October 2014
Pcons+B0034+PBAN(Adoxophyes sp.)+B0034+BFP+J61048
We use this part as reporter gene, it can check whether our PBAN works and quantify its expression.
Fig.1-1 Modeling result of Pcons + RBS + PBAN(AS) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AS) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 903
- 1000COMPATIBLE WITH RFC[1000]