Difference between revisions of "Part:BBa K1438000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer.
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The featured N-terminal His-tag makes it easy for all future iGEM teams to purify the construct using Ni-NTA affinity chromatography.
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+
  
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For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer. We constructed such a improved biobrick <partinfo>BBa_K1438001</partinfo>
  
 
===Source===
 
===Source===

Revision as of 08:51, 17 October 2014


Bacterioferritin (BFR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 142


Design Notes

The featured N-terminal His-tag makes it easy for all future iGEM teams to purify the construct using Ni-NTA affinity chromatography.

For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer. We constructed such a improved biobrick BBa_K1438001

Source

Bacterioferritin from E. coli Nissle 1917 genomic DNA. Amplified by using PCR and cloned into pQE80L

References