Difference between revisions of "Part:BBa K1316017"
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A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein. | A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein. | ||
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| + | <h3> Important note </h3> | ||
| + | There was, unfortunately, a mistake in the design of the reverse primer, which caused the STOP codon of MtrB not to be placed right after the His Tag (where is shoul be) but some base pairs behind. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:35, 17 October 2014
T7 lacO + mtrCAB with His tag
Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis
A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein.
Important note
There was, unfortunately, a mistake in the design of the reverse primer, which caused the STOP codon of MtrB not to be placed right after the His Tag (where is shoul be) but some base pairs behind.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 359