Difference between revisions of "Part:BBa K1431101:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Design the primers and PCR from plasmid.<br> | + | Design the primers and PCR by Q5® High-Fidelity DNA Polymerases from plasmid.<br> |
Primer Forward: T TCTAGA TGGGATCAAGACTGGACAAGA<br> | Primer Forward: T TCTAGA TGGGATCAAGACTGGACAAGA<br> | ||
| − | Primer Reverse: AAAACTGCAG CGGCCGC T ACTAGT A CCGAAGCCCAACCTTTCATA | + | Primer Reverse: AAAACTGCAG CGGCCGC T ACTAGT A CCGAAGCCCAACCTTTCATA<br> |
===Source=== | ===Source=== | ||
Revision as of 08:27, 17 October 2014
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design the primers and PCR by Q5® High-Fidelity DNA Polymerases from plasmid.
Primer Forward: T TCTAGA TGGGATCAAGACTGGACAAGA
Primer Reverse: AAAACTGCAG CGGCCGC T ACTAGT A CCGAAGCCCAACCTTTCATA
Source
The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.