Difference between revisions of "Part:BBa K1431101:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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It is useful for control reaction.
+
Design the primers and PCR from plasmid.<br>
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+
Primer Forward: T TCTAGA TGGGATCAAGACTGGACAAGA<br>
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+
Primer Reverse: AAAACTGCAG CGGCCGC T ACTAGT A CCGAAGCCCAACCTTTCATA
  
 
===Source===
 
===Source===

Revision as of 08:16, 17 October 2014


TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design the primers and PCR from plasmid.
Primer Forward: T TCTAGA TGGGATCAAGACTGGACAAGA
Primer Reverse: AAAACTGCAG CGGCCGC T ACTAGT A CCGAAGCCCAACCTTTCATA

Source

The sequence we got it was from our instructor.

References