Difference between revisions of "Part:BBa K1350007"

Latest revision as of 07:02, 17 October 2014

Cel5A, endoglucanase（BBa_K805011 Improved）

The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Improved endoglucanase Cel5A

BBa_K805011[1] given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly). Because of the degeneracy of codon,the different sequence can encode the same enzyme.This silent mutation makes the part can be compatible with RFC[21].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 307
Illegal NgoMIV site found at 763
Illegal AgeI site found at 1027
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1024

Revision as of 15:16, 15 October 2014 (view source) Yitingluo (Talk \| contribs) (→‎Improved endoglucanase Cel5A) ← Older edit		Latest revision as of 07:02, 17 October 2014 (view source) Timziz (Talk \| contribs) (→‎Improved endoglucanase Cel5A)
Line 6:		Line 6:

	===Improved endoglucanase Cel5A===		===Improved endoglucanase Cel5A===
−	BBa_K805011[https://parts.igem.org/Part:BBa_K805011] given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme.This silent mutation makes the part can be compatible with RFC[21].	+	BBa_K805011[https://parts.igem.org/Part:BBa_K805011] given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly). Because of the degeneracy of codon,the different sequence can encode the same enzyme.This silent mutation makes the part can be compatible with RFC[21].