Difference between revisions of "Part:BBa K1480002"

Latest revision as of 06:13, 17 October 2014

This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.

Usage and Biology

It is a reporter protein that switch off when the repressor TetR binds to ptet.

Results

It is shown in red the relative fluorescence of clones without repressor. The blue bars are for the same clones regulated by the TetR generator K145201. The mean and standard deviation for red bars are 4045 and 226 and for the blue bars are 128 and 2500.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 638
Illegal AgeI site found at 750
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.
-== Usage ==
+== Usage and Biology ==
 It is a reporter protein that switch off when the repressor TetR binds to ptet.
@@ Line 8: / Line 8: @@
 [[File:Ptet_+_mCherry.JPG|thumb|center|400px|It is shown in red the relative fluorescence of clones without repressor. The blue bars are for the same clones regulated by the TetR generator K145201. The mean and standard deviation for red bars are 4045 and 226 and for the blue bars are 128 and 2500.]]
+<!-- -->
+<span class='h3bb'>Sequence and Features</span>
+<partinfo>BBa_K1480002 SequenceAndFeatures</partinfo>