Difference between revisions of "Part:BBa K1480004"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1480004 short</partinfo> | <partinfo>BBa_K1480004 short</partinfo> | ||
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The protein has a LVA tag for degradation. | The protein has a LVA tag for degradation. | ||
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+ | ==Usage and Biology== | ||
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+ | It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter. | ||
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+ | ==Results== | ||
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+ | [[File:TetR repressors(FCB UANL 2014).JPG|thumb|center|400px|It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.]] | ||
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+ | Here are the links for the other parts used. | ||
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+ | https://parts.igem.org/Part:BBa_K1140003 <br> | ||
+ | https://parts.igem.org/Part:BBa_K1480003 <br> | ||
+ | https://parts.igem.org/Part:BBa_K145201 <br> | ||
+ | https://parts.igem.org/Part:BBa_K1480004 <br> | ||
+ | https://parts.igem.org/Part:BBa_K1480005 | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 06:04, 17 October 2014
TetR generator (Promoter J23106 )
This is a TetR protein generator regulated by the constitutive promoter J23106.
The protein has a LVA tag for degradation.
Usage and Biology
It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.
Results
Here are the links for the other parts used.
https://parts.igem.org/Part:BBa_K1140003
https://parts.igem.org/Part:BBa_K1480003
https://parts.igem.org/Part:BBa_K145201
https://parts.igem.org/Part:BBa_K1480004
https://parts.igem.org/Part:BBa_K1480005
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]