Difference between revisions of "Part:BBa K1431101"
Line 2: Line 2:
 
<partinfo>BBa_K1431101 short</partinfo>
 
<partinfo>BBa_K1431101 short</partinfo>
  
−
TetOn-3G(Tetracycline-Controlled Transcriptional Activation) is a system of inducible gene expression systems for mammalian cells.  
+
TetOn(Tetracycline-Controlled Transcriptional Activation,also known as rtTA2<sup>S</sup>-M2) is a system of inducible gene expression systems for mammalian cells. TetOn-3G (also known as rtTA-V16[citation needed]) is similar to TetOn but was derived from rtTA2<sup>S</sup>-S2 rather than rtTA2<sup>S</sup>-M2. The TetOn-3G protein has 5 amino acid differences compared to TetOn which appear to increase its sensitivity to Dox even further. TetOn-3G is sensitive to 100-fold less Dox and is 7-fold more active than the original TetOn.<br>
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:11, 17 October 2014

TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

TetOn(Tetracycline-Controlled Transcriptional Activation,also known as rtTA2S-M2) is a system of inducible gene expression systems for mammalian cells. TetOn-3G (also known as rtTA-V16[citation needed]) is similar to TetOn but was derived from rtTA2S-S2 rather than rtTA2S-M2. The TetOn-3G protein has 5 amino acid differences compared to TetOn which appear to increase its sensitivity to Dox even further. TetOn-3G is sensitive to 100-fold less Dox and is 7-fold more active than the original TetOn.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]