Difference between revisions of "Part:BBa K1323012"
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<partinfo>BBa_K1323012 short</partinfo>
 
<partinfo>BBa_K1323012 short</partinfo>
  
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This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000ug/mL in S.aureus/S.epidermidis hosts (Bose et al., 2013)
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This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000ug/mL in ''S.aureus/S.epidermidis'' hosts (Bose et al., 2013).
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==References==
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Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1323012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1323012 SequenceAndFeatures</partinfo>
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==References==
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Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
  
  

Revision as of 04:58, 17 October 2014

Spc - Spectinomycin Resistance Gene Coding Sequence

This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000ug/mL in S.aureus/S.epidermidis hosts (Bose et al., 2013).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488


References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.