Difference between revisions of "Part:BBa K1323016"
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The Translation Initiation Region ribosome binding site (TIR rbs) was isolated from T7 bacteriophage (Fey, 2014). It is used for optimal expression in s.epidermidis when a native RBS is too poor to achieve desired expression levels. It has a Shine-Dalgarno consensus sequence (AGGAGG). The start codon and the phage T7 enhancer were spaced to optimally minimize secondary structure formation (Cheng et al., 1992). The TIR RBS leads to greater expression levels compared to sodA RBS [[BBa_K1323022]] even though they have the same consensus sequence (Fey, 2014). | The Translation Initiation Region ribosome binding site (TIR rbs) was isolated from T7 bacteriophage (Fey, 2014). It is used for optimal expression in s.epidermidis when a native RBS is too poor to achieve desired expression levels. It has a Shine-Dalgarno consensus sequence (AGGAGG). The start codon and the phage T7 enhancer were spaced to optimally minimize secondary structure formation (Cheng et al., 1992). The TIR RBS leads to greater expression levels compared to sodA RBS [[BBa_K1323022]] even though they have the same consensus sequence (Fey, 2014). | ||
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| + | == References == | ||
Cheng, X., Patterson, T. A., (1992) Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res 20, 4591–4598. | Cheng, X., Patterson, T. A., (1992) Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res 20, 4591–4598. | ||
Revision as of 04:27, 17 October 2014
Translational initiation region (RBS) for Staphylococcus aureus
The Translation Initiation Region ribosome binding site (TIR rbs) was isolated from T7 bacteriophage (Fey, 2014). It is used for optimal expression in s.epidermidis when a native RBS is too poor to achieve desired expression levels. It has a Shine-Dalgarno consensus sequence (AGGAGG). The start codon and the phage T7 enhancer were spaced to optimally minimize secondary structure formation (Cheng et al., 1992). The TIR RBS leads to greater expression levels compared to sodA RBS BBa_K1323022 even though they have the same consensus sequence (Fey, 2014).
References
Cheng, X., Patterson, T. A., (1992) Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res 20, 4591–4598.
Fey, P. D. (2014). Staphylococcus epidermidis: methods and protocols. New York: Springer Science + Business Media, LLC.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]