Difference between revisions of "Part:BBa K1323005:Experience"

Revision as of 04:14, 17 October 2014

sRNAi Silencing assay

YFP construct (pSB3K3 backbone, sarA protomer BBa_K1323021,TIR rbs BBa_K1323016, YFP cdsBBa_K1323010) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.

Applications of BBa_K1323005

User Reviews

UNIQd3a24aeb7e741c2c-partinfo-00000000-QINU UNIQd3a24aeb7e741c2c-partinfo-00000001-QINU

@@ Line 6: / Line 6: @@
 ==sRNAi Silencing assay==
-YFP construct (pSB3K3 backbone, sarA protomer (BBa_K1323021),TIR rbs (BBa_K1323016), YFP cds(BBa_K1323010)) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.
+YFP construct (pSB3K3 backbone, sarA protomer [https://parts.igem.org/Part:BBa_K1323021 BBa_K1323021],TIR rbs [https://parts.igem.org/Part:BBa_K1323016 BBa_K1323016], YFP cds[https://parts.igem.org/Part:BBa_K1323010 BBa_K1323010]) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.
 ===Applications of BBa_K1323005===