Difference between revisions of "Part:BBa K1323012"
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This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000ug/mL in S.aureus/S.epidermidis hosts (Bose et al., 2013)
 
This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000ug/mL in S.aureus/S.epidermidis hosts (Bose et al., 2013)
  
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==References==
  
 
Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
 
Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 04:11, 17 October 2014

Spc - Spectinomycin Resistance Gene Coding Sequence

This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000ug/mL in S.aureus/S.epidermidis hosts (Bose et al., 2013)


References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488